According to the survey, most of the microbial fertilizer products used in China have been produced using spore-free strains. Since the spore-free species are generally not resistant to high temperature and dryness, and are not resistant to salt damage, the stress resistance is low, the adaptability is poor, the storage period is short, and it is difficult to form an industrialization. A. aeruginosa is a group of bacteria with strong resistance and wide adaptability in the soil microflora. Many of them have strong enzyme systems and can actively participate in the transformation of various nutrients in the soil. They are the main decomposers of a group of relatively stable organic matter. Therefore, the development and utilization of Bacillus domesticus is becoming more and more active, and some new products (bio-potassium fertilizer, bio-phosphorus fertilizer and biological fertilizers such as organic or inorganic and microbial complexes) are constantly emerging. However, the survival rate of Bacillus in high-salt (NPK) matrix and high-temperature granulation has not been reported. To this end, we conducted experiments on the effects of different fertilizer substrates and production processes on the survival rates of three Bacillus strains. The results showed that the survival rate of Bacillus cereus in the purified fertilizer was the lowest, about 50-70% after storage for more than one year. In the organic-inorganic composite matrix (NPK=12%), the survival rate was about 70-90%; while in the other four matrices (without fertilizer), the survival rate is more than 90%. The survival rate of Bacillus licheniformis in substrates with low fertilizer content (NPK = 16%) was higher in the medium with higher fertilizer content (NPK = 18%), while the survival of Bacillus polymyxa in both substrates The rates are similar. However, in the same matrix, the survival rate of B. polymyxa is much higher than that of Bacillus licheniformis. Tests on different production granulation processes have shown that the survival rate of Bacillus polymyxa using the roll granulation is about 70%, while the survival rate by the disc granulation drying process is only 0.4%. It shows that high temperature drying has a great effect on the inactivation of Bacillus. Therefore, it is important to select suitable production processes and supporting equipment for the production of fertilizers containing effective microorganisms.
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